Department of Microbiology

Faculty and Staff

Name Designation Unit
Prof. (Dr.) Banya Chakraborty Professor and Head
Prof. (Dr.) Munmun Das (Sarkar) Professor Protozoology Unit (In-Charge)
Prof. (Dr.) Tapashi Ghosh Professor Helminthology Unit (In-Charge)
Prof. (Dr.) Bhaswati Bandyopadhyay Professor Virology Unit (In-Charge)
Dr. Ardhendu Kumar Maji Reader Protozoology Unit & Entomology Unit (In-Charge)
Dr. Sampurna Biswas Pramanik Associate Professor Mycology Unit (In-Charge)
Dr. Manjula Dutta Assistant Professor Mycology Unit
Dr. Anindita Rakshit Assistant Professor Bacteriology Unit
Dr. Abhishek Sengupta Assistant Professor Helminthology Unit
Dr. Srima Adhikari RMO cum Clinical Tutor Virology Unit
Dr. Shubhasree Halder Demonstrator Virology Unit
Dr. Raja Mukherjee Medical Officer Helminthology Unit
Dr. Bishal Gupta Senior Resident Virology Unit
Dr. Malabika Biswas Senior Resident Bacteriology Unit
Dr. Aitihya Chakraborty Senior Resident Protozoology Unit
Dr. Sonia Deb Senior Resident Virology Unit
Dr. Alisha Acharya Senior Resident Virology Unit
Dr. Sayani Bose Senior Resident Virology Unit
Dr. Sudip Ballav Project Fellows Entomology
Anwesha Samanta Project Fellows Protozoology
Sunanda Ghosh Project Fellows Protozoology
Ms. Risha Das Project Insect Collector Entomology
Banamali Mandal Project MT Lab Protozoology
Tanmay Das Project MTS Protozoology
Aparna Chowdhury Molecular Biologist, COVID Virology
Kanchan Biswas Molecular Biologist, COVID Virology
Hemajuddin Momin Project MTS Protozoology
Anurita Mukherjee Counselor, (ICTC) Virology
Amrita Roy Counselor, (ICTC) Virology
Amit Kumar Maji Medical Technologist (Lab) Bacteriology
Trisha Mondal Medical Technologist (Lab) Bacteriology
Golam Dostogir Medical Technologist (Lab) Bacteriology
Manas Mallik Medical Technologist (Lab) Bacteriology
SK Monirul Islam Medical Technologist (Lab) Bacteriology
Sukdev Ganguly Medical Technologist (Lab) Bacteriology
MD Ruhul Amin Medical Technologist (Lab) Bacteriology
Sampa Sadhu Medical Technologist (Lab) Bacteriology
S. J. Ansari Medical Technologist (Lab) Mycology
Partho Sengupta Medical Technologist (Lab) Mycology
Subhas Chandra Mondal Medical Technologist (Lab) Helminthology
Bijay Nath Medical Technologist (Lab) Helminthology
Himadri Sekhar Mondal Medical Technologist (Lab) Helminthology
Samit Kumar Mondal Medical Technologist (Lab) Protozoology
Supriya Halder Medical Technologist (Lab) Protozoology
Shrabani Nath Medical Technologist (Lab) Protozoology
Paramita Das Adhikary Medical Technologist (Lab) Protozoology
Santasree Chakraborty Medical Technologist (Lab) Virology
Sutapa Karmakar Medical Technologist (Lab) Virology
Kaushik Karan Medical Technologist (Lab) Virology
Bappaditya Pan Medical Technologist (Lab) Virology
Atanu Guchhait Medical Technologist (Lab) Virology
Rony Roy Chowdhury Medical Technologist (Lab) Virology
Joydeep Roy Medical Technologist (Lab) Virology
Balaram Barik Medical Technologist (Lab), COVID Bacteriology
Dhiman Chandra Das Medical Technologist (Lab), COVID Virology
Subhankar Sikder Medical Technologist (Lab), COVID Virology
Ashis Kumar Paul Laboratory Technologist, (ICTC) Virology
Suman Mohan Chowdhury Laboratory Technologist, (ICTC) Virology
Surya Kumar Haldar Laboratory Technologist, (NRL) Virology
Saiantani Mondal Technical Officer, (NRL) Virology
Farhin Ara Project Co-Ordinator, Covid Project Bacteriology
Goutam Roy Glass Blower Bacteriology
Trisha Sarkar Pharmacist, National AMR Surveillance Programme Bacteriology
Sudipta Som Laboratory Technician, National AMR Surveillance Programme Bacteriology
Avik Maity Data Manager, National AMR Surveillance Programme Bacteriology
Sujay Gupta Executive Assistant (IDSP)
Narayan Nandi G.D.A. Bacteriology
Sikandar Ram G.D.A. Helminthology
Miran Hazra CT & SW Bacteriology
Ram Saran Mallick CT & SW Bacteriology
Sanjay Hela CT & SW Entomology
K.Narsimha Rao CT & SW Helminthology
Anita Kanungo CT & SW Mycology
Bhola Hela CT & SW Protozoology
Ramesh Paul CT & SW Virology
Jitendra Hela CT & SW Virology
Bivas Sardar House Keeping Staff Helminthology
Madhusudan Dey House Keeping Staff Protozoology
Md. Saddam House Keeping Staff Virology
Bibhas Sardar House Keeping Staff Helminthology
Shouvanik Dey Data Entry Operator, COVID Virology

Academic Course

MD (Microbiology)

Patient Care Services

Bacteriology and Serology Unit

Annual investigation report for the year 2022 (1st Jan to 31st Dec)

Investigation Number
Gram Staining 2529
ZN Staining 1920
Albert Staining 591
Urine 2842
Sputum 831
Blood 1190
Miscellaneous(All body fluids,Pus, Wound swab) 1007
Throat Swab 52
NP Swab for Pertussis 175
Throat Swab for Diptheria 157
ABST 1277
Typhi DOT 299
ASO 11
Brucella IgM 348
Toxoplasma IgG 365
Pertussis IgG 434
Diptheria PCR 157
Pertussis PCR 175


Protozoology Unit

Routine clinical & diagnostic service to the patient - the unit provides diagnostic & clinical service to the patient throughout the year on Malaria, Kala-azar and other protozoan infections. It also serves as a referral unit for protozoan infections.

Incidence of malaria detected and treated (2022)

Total No. of patients Examined P. vivax P. falciparum Mixed Total Positive SPR
10322 2146 1037 98 3281 31.79

Routine diagnostic service to the patients of Kala-azar / rk-39 test (2022)

Total Examined Total Positive % of Positive
244 8 3.28

Entomology Unit

Daily activities:

  1. Entomological survey of dengue vectors
  2. Maintenance of Aedes sp. colony
  3. Insecticide susceptibility bioassay of adult and larvae of mosquito vectors

Helminthology Unit

Filaria OPD attendance (2022): 1656

Unit Laboratory (2022)

Laboratory Investigation Number
Circulating Filaria Antigen Test 362
Differential Count 341
Filaria Concentration Technique 321
Stool Concentration Technique 1626
Modified ZN Stain 209

Mycology Unit

Number of investigations (2022)

KOH Preparation India Ink Preparation Culture Total Sample
435 134 460 629

Virology Unit

Routine Activities of Virology Unit

  • Arbo viruses
    • Serological diagnosis of
      1. Dengue NS1 detection by ELISA
      2. Anti-Dengue IgM ELISA
      3. Anti-Dengue IgG ELISA
      4. Anti-Chikungunya IgM ELISA
      5. Anti-Japanese encephalitis IgM ELISA
    • Molecular Diagnosis of
      1. Chikungunya virus RNA detection
      2. Dengue virus RNA detection
      3. Dengue serotyping
  • Hepatitis viruses
    • Serological diagnosis of
      1. Anti-HCV antibodies ELISA / RAPID
      2. Hepatitis B s antigen ELISA/ RAPID
      3. Anti-HAV IgM ELISA
      4. Anti-HEV IgM ELISA
      5. Hepatitis B e antigen ELISA
      6. Anti HBs antibody titre ELISA
      7. Anti HBc Antibody ELISA
  • Human Immune Deficiency Virus (HIV)
    • Serological diagnosis of
      1. HIV 1 and 2 by RAPID, ELISA and Western blot test (as per NACO guideline)
    • ICTC - Routine testing of HIV among patients referred from STI clinic
    • NRL -
      1. Conducts proficiency testing and panel sera distribution (HIV EQAS) to the HIV State Reference Laboratories (SRL) of West Bengal, Bihar, Sikkim and Chhattisgarh
      2. Confirmation of Indeterminate and discordant samples by Western Blot test
      3. Confirmation of HIV 2 by Western Blot test (NRL is also designated as Referral Lab for the states of West Bengal, Bihar, Sikkim and Jharkhand for HIV 2 diagnosis)
  • SARS CoV2
    1. Molecular diagnosis (RT-PCR) - Almost 100000 (One lac) SARS CoV-2 RT-PCR test has been done.
    2. Rapid Antigen Test (Lateral flow assay)
    3. SARS CoV 2 IgG by ELISA testing
    4. Regular QC test of different RAT kit, RNA Extraction kit and RT-PCR kit done.
    5. Acting as State nodal Center for Whole Genome Sequencing of SARS CoV-2 virus.
  • Acute Encephalitis Syndrome (AES) Panel (other than Arbo viral causes)
    • Molecular testing of
      1. Scrub typhus
      2. Herpes Simplex Virus 1 and 2
      3. Leptospira
      4. Streptococcus pneumoniae
      5. Hemophillus influenzae
      6. Neisseria meningitides
    • Serological testing (IgM) of
      1. Scrub typhus
      2. Herpes Simplex Virus 1 and 2
      3. Leptospira
  • Acute Respiratory Infection (ARI) Panel
    • Molecular testing of
      1. Influenza A + B Flu typing
      2. Sub typing of Influenza A H1N1, pdm009,H3N2
      3. Para Influenza (1,2,3&4)
      4. Respiratory syncytial virus (RSV)
      5. Adeno virus
      6. Entero virus
      7. Rhino virus
      8. Human Metapneumo virus (HMPV)
      9. Human Boca Virus
  • Others
    • Epstein Barr virus (EBV)
    • Cytomegalo virus (CMV)
  • Next Generation Sequencing

Since January 2020, Virology unit is designated as one of the State Reference Laboratories of NVHCP programme for several Health districts of West Bengal.

Yearly testing details (2022)

Name of the Test Total No. of Patient Tested
Dengue NS1 detection by ELISA 1570
Anti-Dengue IgM ELISA 1679
Anti-Chikungunya IgM ELISA 794
Anti-Japanese encephalitis IgM ELISA 595
Anti-Scrub typhus IgM ELISA 2464
Anti-Leptospira IgM ELISA 1754
Anti-Herpes Simplex IgM ELISA 16
Hepatitis B s antigen ELISA/ RAPID 5103
Anti-HCV antibodies ELISA / RAPID 5004
Anti-HAV IgM ELISA 776
Anti-HEV IgM ELISA 776
Hepatitis B e antigen ELISA 648
HIV antibodies Rapid 7400
Anti-HIV antibodies by Western blot tests 21
Anti Hbs antibody titre 159
Anti Hbc Antibody 67
SARS CoV-2 RT-PCR 75267
Influenza A + B Flu typing and H1N1pdm009, H3N2, H5N1, H7N9 sub typing virus detection by Real-time RT PCR 671
Dengue Serotype RT-PCR 268
CMV 44
Scrub 23
JE 13
Leptospira 28
Dengue 15
Chikungunya 25
HSV 57
JC & BK 2

Ongoing Research Projects

Investigator Title of the project
Dr. A.K.Maji Studies on Population Dynamics and Insecticide Susceptibility Status of Dengue Vectors in Bidhan Nagar Municipal Corporation Areas, Kolkata, West Bengal
Prof. (Dr.) S.K.Guha, Dr. A.K.Maji Prevention of Resurgence of Leishmaniasis during Post Elimination Period in Erstwhile Endemic Pockets of West Bengal
Prof. (Dr.) B. Bandyopadhyay, Prof. (Dr.) S.K.Guha Development of therapeutic broadly neutralizing monoclonal antibodies against circulating HIV-1 clade C in children and adults
Prof. (Dr.) B. Bandyopadhyay, Prof. (Dr.) S.K.Guha Developing broadly neutralizing monoclonal antibody mediated prevention and treatment strategy by assessing their effectiveness in neutralizing HIV-1 subtype C circulating in India across different regions and distinct risk groups
Prof. (Dr.) B. Saha, Prof. (Dr.) B. Bandyopadhyay, Dr Sudeshna Mallik, Dr Sumi Mukhopadhyay, Dr Indranil Dhar Development of a simple scoring system based on clinical findings and simple laboratory tests for patients presenting with acute febrile illness

Recent Publications

  1. Biswas M,Gupta B,Rakshit A,Pramanik N,Chakrabarty B,Kundu PK.The bane of enterobacterales in hospital infection control: a tertiary carebased study.European Journal Of Pharmaceutical And Medical Research, 2022; 9(1), 302-306.
  2. Biswas M, Biswas, Gupta B, Mascellino M, Rakshit A, Chakraborty Banya. Changing Paradigms in Antibiotic Resistance in Salmonella Species with Focus on Fluoroquinolone Resistance: A 5-Year Retrospective Study of Enteric Fever in a Tertiary Care Hospital in Kolkata, India. Antibiotics. 2022. 11. 1308. 10.3390/antibiotics11101308.
  3. Sikdar Rahul, Gupta B, Chakraborty D, Chakraborti R, Chowdhury A, Mondal S, Chakraborty B, Bandyopadhyay B. A Clinico-Epidemiological Study on Leptospirosis in West Bengal: A Neglected Cause of Acute Febrile Illness. Paripex Indian Journal of Research. 2022. 42-45. 10.36106/paripex/1401432.
  4. Sardar AA, Saha P*, Maji D, Guha U, Maji AK, Guha SK. Impact of Indoor Residual Spray (IRS) of synthetic pyrethroid (alphacypermethrin) on sand fly population in the Kala-azar endemic areas of Malda district, West Bengal, India. International Journal of Tropical Insect Science. 2022. 42, 3290-3302.
  5. Chatterjee R, Pramanick N, Ghosh S*, Ghosh T, Mondal T, Maji A. Multiple protozoal infections in a single immunocompromised patient – A case report. Indian Journal of Tropical Parasitology. 2022.
  6. Bose S, Chakrabarty A*, Ghosh T, Maji A. Rare Presentation of Ocular Enterobiasis-Case Reports. Indian Journal of Tropical Parasitology. 2022.
  7. Haldar R, Das (Sarkar) Munmun, An Observational Study on Asymptomatic Bacteriuria in Pregnant Women Attending Antenatal Clinic in a Tertiary Care Hospital. Journal of The West Bengal University of Health Sciences, October 2022, Vol 3 Issue 2: 05-12
  8. Dey A, Biswas AK, Jana K, Maji AK, Saha P*. Assessment of knowledge about malaria and LLIN ownership and its use in Bankura, West Bengal. Journal of Vector Borne Diseases (In Press). 2022
  9. Acharya A, Saha P, Chaudhury A, Guha SK, Maji AK*. Prevalence of histidine‑rich protein 2 deletion among the clinical Plasmodium falciparum isolates collected from urban area, Kolkata. Tropical Parasitology (In Press).2022.
  10. Sikdar R, Gupta B, Chakraborty D, Chakraborti R*, Chowdhury A, Mondal S, Chakraborty B, Bandyopadhyay B. A clinico-epidemiological study on leptospirosis in west bengal: a neglected cause of acute febrile illness, Indian Journal of Research. October 2022 Volume - 11 | Issue - 10 | 2 | PRINT ISSN No. 2250 - 1991 | DOI : 10.36106/paripex
  11. Ravi V, Hameed SKS, Desai A, Mani RS, Reddy V, Velayudhan A, Yadav R, Jain A, Saikia L, Borthakur AK, Sharma A, Mohan DG, Bandopadhyay B, Bhattacharya N, Inamdar L, Hossain S, Daves S, Sejvar J, Dhariwal AC, Sen PK, Venkatesh S, Prasad J, Laserson K, Srikanti P. An algorithmic approach to identifying the aetiology of acute encephalitis syndrome in India: results of a 4-year enhanced surveillance study. Lancet Glob Health. 2022 May;10(5):e685-e693. doi: 10.1016/S2214-109X(22)00079-1.
  12. ChitraP,Pramada Prasad, SampadaSudarshan, Anson George, Darshan S, Risha Ashraf, Ayushman Ghosh, Ananya Pal, ,Shafeeq K, Shahul Hameed, Bhaswati Banerjee, Anita desai, Ravi V. Identification and genomic characterization of Parvovirus B19V genotype 3 viruses from cases of meningoencephalitis in West Bengal, India. has been accepted by Spectrum. MicrobiolSpectr 2022 Apr 27;10(2):e0225121. doi: 10.1128/spectrum.02251-21. Epub 2022 Apr 12
  13. Singh AK, Laskar R, Banerjee A, Mondal RK, Gupta B, Deb S, Dutta S, Patra S, Ghosh T, Sarkar S, Ghosh S, Bhattacharya S, Roy D, Chakraborty A, Chowdhury M, Mahaptra S, Paul A, Mazumder A, Chowdhury A, Chatterjee SS, Sarkar A, Ray R, Pal K, Jana A, Barik G, Ganguly S, Chatterjee M, Majhi D, Bandopadhyay B, Das S, Maitra A, Biswas NK. Contrasting Distribution of SARS-CoV-2 Lineages across Multiple Rounds of Pandemic Waves in West Bengal, the Gateway of East and North-East States of India. American Society for Microbiology. 19 July 2022 by 2405:201:8013:f9d0:dc28:b9c8:4419:2761.
  14. Deb S, Chattopadhyay S, Gupta B, Chakraborti R, Guha SK, Bandyopadhyay B. Evaluation of Antigen Based Rapid Diagnostic Test in Comparison to RT PCR in Diagnosis of SARS CoV2 with Respect to Duration of Illness and CT Value of Corresponding RT PCR. Accepted For Publication 2.9.2022 International Journal Of Health And Clinical Research
  15. Bose S, Palchowdhury P, Acharya A, Chakraborti R*, Bandyopadhyay B, Mandal S. Influenza amidst The Covid-19 Pandemic : A Declining Trend, International Journal Of Scientific Research, Volume 11, Issue – 05, May – 2022, PRINT ISSN No. 2277 - 8179 | DOI : 10.36106/ijsr
  16. Chakraborty A, Rakshit A, Palchowdhury P, Chakraborty B*. High Prevalence of Fluoroquinolone Resistance among Drug-Sensitive Pulmonary Tuberculosis Patients: An Imminent Peril. Global journal of Research Analysis. Apr 1, 2022